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Thermo Fisher
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Journal: Journal of Neurochemistry
Article Title: Targeting Glutamate Excitotoxicity With Memantine Modulates Glial Response and Protects Motoneurons After Spinal Root Lesion
doi: 10.1111/jnc.70429
Figure Lengend Snippet: Relative expression of genes involved in glutamatergic signaling in the different experimental groups ( n = 4/5 mice per group). The graphs demonstrate the analysis of the relative expression of the genes (A) Glast , (B) Glt1 , (C) Glul , (D) Gria2 . There was no significant difference in the expression of these genes after injury or treatment (one‐way ANOVA followed by Tukey's post‐test; mean ± SEM).
Article Snippet: Glast (Slc1a3) ,
Techniques: Expressing
Journal: PLOS Genetics
Article Title: Plagl1 regulates the retinal progenitor cell to Müller glial cell transition
doi: 10.1371/journal.pgen.1012020
Figure Lengend Snippet: (A) Establishment of a conditional transgenic approach to activate gene expression in perinatal/postnatal Müller glia using Slc1a3-CreERT driver mice and a Rosa-zsGreen reporter line. (B) 4-OHT treatment of Slc1a3-CreERT;Rosa-zsGreen mice at P5, P7 and P14, followed by analysis of the distribution of zsGreen + cells at P21. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 50µm. (C) Targeting strategy to generate a floxed allele of Plagl1 (from ), and crosses to Slc1a3-CreERT to generate a Müller glia specific Plagl1 -cKO. Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/gjcg2ar (D) Tamoxifen injections were administered at P14 every second day and animals were assessed at P21. Sox9 and zsGreen immunostaining in P21 Slc1a3-CreERT ; Rosa-zsGreen control and Slc1a3-CreERT;Plagl1 floxed retinas (i.e., Plagl1 -cKOs). Schematics created in BioRender. Schuurmans, C. (2025). https://BioRender.com/n6p34rb . Scale bars: 25µm. (E) Total zsGreen + (ZsG + ) and Sox9 + cells were quantified per field. Mean values and error bars representing s.e.m. were plotted. p-values calculated with unpaired t-test.
Article Snippet: Plagl1 fl/+ males (received from Pengpeng Bi) [ ] were crossed with
Techniques: Transgenic Assay, Gene Expression, Immunostaining, Control